Duke Neurobiology
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Laboratory of Ryohei Yasuda, Ph.D.MainLab PersonnelRecent Papers
Selected Publications:
*Equal contribution, +Correspondence
C.D.Harvey*, R.Yasuda*+, H.Zhong, K.Svoboda+ (2008) The spread of Ras activity triggered by activation of a single dendritic spine. Science 10.1126/science.1159675 (Science express)

H.Murakoshi, S.-J.Lee, and R.Yasuda (2008) Highly sensitive and quantitative FRET-FLIM imaging in single dendritic spines using improved non-radiative YFP. Brain Cell Biol. DOI: 10.1007/s11068-008-9024-9
M.C.Fischer, H.C.Liu, I.R.Piletic, Y.Escobedo-Lozoya, R.Yasuda, and W.S.Warren (2008) Self-phase modulation signatures of neuronal activity. Opt. Lett. 33: 219-21
R.Yasuda (2006) Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy. Curr. Opin. Neurobiol. 16: 551-61

R.Yasuda, C.D.Harvey, H.Zhong, A.Sobczyk, L.van Aelst and K.Svoboda (2006) Super-sensitive Ras activation in dendrites and spines revealed by 2-photon fluorescence lifetime imaging. Nat. Neurosci. 9: 283-291.
K.Svoboda and R.Yasuda (2006) Principles of two-photon excitation microscopy and its applications to neuroscience Neuron 50, 823–839.
V.Scheuss, R.Yasuda, A.Sobczyk and K.Svoboda (2006) Nonlinear [Ca2+] Signaling in Dendrites and Spines Caused by Activity-Dependent Depression of Ca2+ Extrusion. J. Neurosci. 26: 8183-8194.

R.Yasuda, E.A.Nimchinsky, V.Scheuss, T.A.Pologruto, T.G.Oertner, B.Sabatini, K.Svoboda (2004) Imaging calcium concentration dynamics in small neuronal compartment. Science's STKE pl5, 10.

E.Nimchinsky, R.Yasuda, T.Oertner and K.Svoboda (2004) The number of glutamate receptors opened by synaptic stimulation in single hippocampal spines. J. Neurosci. 24: 2054-2064.
H.Itoh, A.Takahashi, K.Adachi, H.Noji, R.Yasuda, M.Yoshida and K.Kinosita,Jr. (2004) Mechanically driven ATP synthesis by F1-ATPase. Nature 427: 465-468.

T.Pologruto, R.Yasuda and K.Svoboda (2004) Monitoring neural activity and [Ca2+] with genetically encoded Ca2+ indicators. J. Neurosci. 24:9572-9579.

R.Yasuda, B.L.Sabatini and K.Svoboda (2003) Plasticity of calcium channels in dendritic spines. Nat. Neurosci. 6: 948-955.
R.Yasuda, T.Masaike, K.Adachi, H.Noji, H.Itoh, K.Kinosita,Jr. and M.Yoshida (2003) The ATP-waiting conformation of rotating F1-ATPase revealed by single-pair fluorescence resonance energy transfer. Proc.Natl.Acad.Sci.USA 100: 9314-9318.

R.Yasuda, H.Noji, M.Yoshida, K.Kinosita,Jr. and H.Itoh (2001) Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase. Nature 410 : 898-904.
Y.Arai, R.Yasuda, K.Akashi, H.Miyata, K.Kinosita, Jr., Y.Harada, and H.Itoh (1999) Tying a molecular knot with optical tweezers. Nature 399:446-448.

R.Yasuda*, H.Noji*, K.Kinosita,Jr., and M.Yoshida (1998) F1-ATPase is a highly efficient motor that rotates with discrete 120-degree steps. Cell 93:1117-1124.
K.Kinosita,Jr., R.Yasuda, H. Noji, S. Ishiwata, and M. Yoshida (1998) F1-ATPase: a rotary motor made of a single molecule. Cell 93: 21-24.

H.Noji*, R.Yasuda*, M. Yoshida, and K. Kinosita, Jr. (1997) Direct observation of the rotation of F1-ATPase. Nature 386:299-302.