The TRP superfamily of ion channels exhibit a diverse selectivity to various cations and hence diverse biophysical properties and ion gating mechanisms. My research is geared towards how structural motifs in TRP(V) channels affect their function in various cell types, both excitable and non-excitable. I primarily focus on how region-specific alterations of the channel, from mutation of motifs going down in resolution to the single amino acid, affect calcium permeability of the channel as well as membrane potential of the cell. For these structure-function studies. I am utilizing Calcium imaging coupled with patch clamp recordings.