Combating pain is at the core of patient care provided by medical doctors. Pain can have many facets including, by origin, inflammatory pain, neuropathic pain, cancer pain, and by locale, joint, back, head (migraine), trigeminal pain and others. In vertebrates, specialized sensory neurons, "nociceptors", transduce the painful stimulus from peripheral. My project is primarily concerned with the role of primary sensory neurons in pain, and with the role of the TRPV4 ion channel in pain transduction and transmission. Forms of pain that I specifically investigate include trigeminal pain and irritation, using molecular neurophysiology methods focused on channel function in conjunction with behavioral assessment.