Hull Research

Research Strategy

To reveal how cerebellar circuits enable the coordination of body movements, we are taking advantage of a wide range of modern techniques, including:

  • High speed resonant scanning 2-photon imaging in vivo
  • Whole-cell electrophysiology (current and voltage-clamp recordings)
  • Intrinsic imaging of macroscopic neural activity patterns
  • Viral expression of calcium indicators and other fluorophores
  • Behavioral studies of cerebellar-dependent processing related to movement coordination
  • Single and multi-unit extracellular recordings in vivo

By combining these approaches, both in vivo and in vitro, it is out goal to understand cerebellar circuit function at levels spanning from single cells and subcellular processes up to whole animal behaviors.

In vivo widefield calcium imaging in awake, behaving mice

Visualizing neural population dynamics across millimeters of cerebellar cortex at low magnification in vivo during behavior as a tool to study the mechanisms that underlie motor learning.

GCaMP6f expressed in Purkinje cells

Resonant scanning 2-photon imaging in vivo

Using a high speed, resonant scanning microscope we can zoom in at high magnification to visualize individual dendrites and changes in calcium signals corresponding to synaptic inputs during motor learning

GCaMP6f expressed in Purkinje cells