Duke Neurobiology's Jorg Grandl, PhD and Amanda H. Lewis of Grandl Lab have a new publication in eLIFE titled, "Mechanical sensitivity of Piezo1 ion channels can be tuned by cellular membrane tension."
Publication Abstract: Piezo1 ion channels mediate the conversion of mechanical forces into electrical signals and are critical for responsiveness to touch in metazoans. The apparent mechanical sensitivity of Piezo1 varies substantially across cellular environments, stimulating methods and protocols, raising the fundamental questions of what precise physical stimulus activates the channel and how its stimulus sensitivity is regulated. In the publication, Grandl Lab measured Piezo1 currents evoked by membrane stretch in three patch configurations, while simultaneously visualizing and measuring membrane geometry. Building on this approach, they developed protocols to minimize resting membrane curvature and tension prior to probing Piezo activity. They find that Piezo1 responds to lateral membrane tension with exquisite sensitivity as compared to other mechanically activated channels and that resting tension can drive channel inactivation, thereby tuning overall mechanical sensitivity of Piezo1. Their results explain how Piezo1 can function efficiently and with adaptable sensitivity as a sensor of mechanical stimulation in diverse cellular contexts.